Journal
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Immunologie et infection
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协同刺激信号封锁的同种抗原刺激诱导人外周血单个核细胞同种异体抗原特异性无能
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade
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协同刺激信号封锁的同种抗原刺激诱导人外周血单个核细胞同种异体抗原特异性无能

DOI:
10.3791/2673-v

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11:55 min

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March 14, 2011

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, , , ,
1Medical Oncology,Dana Farber Cancer Institute, 2Department of Medicine,Brigham and Womens Hospital, 3Pediatric Oncology,Dana Farber Cancer Institute, 4Division of Hematology/Oncology,Children’s Hospital Boston

Chapitres

  • 00:05Titre
  • 02:00Preparation of PBMC and Bulk Alloanergizing Cultures
  • 03:57Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
  • 06:48Measuring the Specificity of Alloanergizing
  • 09:05Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
  • 09:43Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
  • 10:34Conclusion

Summary

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本文介绍了一个简单的方法诱导人外周血单个核细胞同种异体抗原特异性无能。该技术可应用于临床，产生非同种异体的供体细胞。这些细胞输注可以提高免疫功能重建和减少异基因造血干细胞移植后的毒性。

Tags

Alloantigen-specific AnergyInductionHuman Peripheral Blood Mononuclear CellsAlloantigen StimulationCo-stimulatory Signal BlockadeAllogeneic Hematopoietic Stem Cell TransplantationGraft-versus-Host DiseaseAHSCTDonor T CellsTransplantationImmune ReconstitutionRelapse PreventionNon-specific DepletionPharmacological ImmunosuppressionAllodepletion StrategiesRecipient Antigen Presenting CellsAllodepletion TechniquesOff-target EffectsInactivation

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