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Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
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Journal JoVE Neurosciences
Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

DOI:
10.3791/2681-v

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07:29 min

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May 25, 2011

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, , , ,
1Department of Neurology,Beth Israel Deaconess Medical Center, 2Department of Obstetrics and Gynecology,Brigham and Women’s Hospital, 3Department of Pathology,Beth Israel Deaconess Medical Center, 4Department of Pathology, Division of Neuropathology,Brigham and Women’s Hospital

Chapitres

  • 00:05Titre
  • 01:13Preparation of Solutions and Materials
  • 02:08Isolating Human Neural Stem Cells from the Human Fetal Brain
  • 04:27Differentiating or Storing the Neural Stem Cells
  • 06:23Transfected and Differentiated Neurospheres
  • 06:53Conclusion

Summary

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Traduction automatique

A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.

Tags

Neural Stem CellsNSCsNeuroepitheliumCortical PlateIntermediate ProgenitorsNeuronal CellsGlial CellsCerebral CortexNormal Human NSC DevelopmentNeurological DisordersProgenitor ProliferationMigrationDifferentiationDown Syndrome NSCsAlzheimer’s Disease PhenotypeGenes Located On Human Chromosome 21DissectionCell SortingPlatingPassaging Of Human NSCsDifferentiation Of Human NSCs

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