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An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
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Journal JoVE Neurosciences
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

DOI:
10.3791/2685-v

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09:07 min

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April 25, 2011

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, , , , , , ,
1National Eye Institute,NIH, 2Ophthalmology Department,The Second Hospital of Harbin Medical University

Chapitres

  • 00:05Titre
  • 02:25Baseline Retrograde Labeling of Retinal Ganglion Cells (RGCs) at Day 1
  • 04:47Optic Nerve Crush Injury at Day 4 after Fluorescence Dye Injection
  • 06:22Retinal Harvest and Analysis of RGC Survival
  • 07:13Analysis of Retinal Ganglion Cell Survival after Optic Nerve Crush Injury
  • 08:01Conclusion

Summary

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This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.

Tags

Optic Nerve Crush InjuryRetinal Ganglion Cell SurvivalOptic NeuropathyGlaucomaVision LossIrreversibleExperimental Disease ModelApoptosisNeuronal Death And SurvivalTherapeutic MeasuresTherapeutic ReagentsRetrograde LabelingHarvest The RetinaeRGC Survival

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