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Chapitres
- 00:05Titre
- 01:58Egg Collection and Dechorionation
- 02:34Microinjection with GDP-FucAI
- 03:30BTTES-Cu(I)-catalyzed Click Chemistry Reaction
- 04:34Imaging
- 05:06In Vivo Imaging of Fucosylated Glycans During Zebrafish Embryogenesis
- 06:11Conclusion
A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.
Tags
GlycansZebrafish EmbryosMetabolic LabelingBioorthogonal Click ChemistryChemical Reporter StrategyAzide-tagged MonosaccharidesAlkyne-tagged MonosaccharidesGlycan Biosynthetic MachineryCell Surface GlycoconjugatesFluorescent ProbesAffinity ProbesNoninvasive ImagingFucosylated GlycansMicroinjectionGDP-5-alkynylfucose (GDP-FucAl)Azide-conjugated FluorophoresCu(I)-catalyzed Azide-alkyne Cycloaddition (CuAAC)Confocal MicroscopySialic Acid-containing GlycansN-acetylgalactosamine-containing Glycans
