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Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
Journal JoVE
Biologie
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Journal JoVE Biologie
Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

DOI:
10.3791/2717-v

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10:19 min

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June 30, 2011

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, , , , ,
1Department of Physiology and Biophysics,Robert Wood Johnson Medical School

Chapitres

  • 00:05Titre
  • 01:25Treadmill Running
  • 03:12Membrane Repair Capacity of Isolated Muscle Fibers Following UV-Laser Damage
  • 07:25Live Cell Confocal Imaging to Monitor the Dynamic Process of Cell Membrane Repair
  • 08:41Reduced Running Capacity and Compromised Membrane Repair Capacity
  • 09:53Conclusion

Summary

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Described here are protocols used to visualize the dynamic process of MG53-mediated cell membrane repair in whole animals and at the cellular level. These methods can be applied to investigate the cell biology of plasma membrane resealing and regenerative medicine.

Tags

MG53Cell Membrane RepairIn Vivo SystemIn Vitro SystemAcute InjuryCellular PhysiologyDefective Membrane RepairDegenerative Human DiseasesMolecular UnderstandingRegenerative MedicineExperimental ProtocolsMuscle InjuryTreadmill Exercise ProtocolsMouse ModelsEx Vivo Membrane Repair CapacityDye EntryIsolated Muscle FibersVesicle TraffickingLive Cell Confocal Microscopy

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