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一个简单的悬滴细胞生成三维球体文化协议

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Bio-ingénierie

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Journal JoVE Bio-ingénierie

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

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一个简单的悬滴细胞生成三维球体文化协议

DOI:

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07:24 min

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May 06, 2011

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Ramsey Foty

¹Department of Surgery,UMDNJ-Robert Wood Johnson Medical School

Chapitres

00:05Titre
01:14Preparing a Single Cell Suspension
02:57Hanging Drop Formation
04:38Representative Results of Hanging Drop Cultures, Compaction of Cellular Aggregates and Spheroid Bodies
07:12Conclusion

Summary

Traduction automatique

我们描述了一个简单快速的方法，生成三维组织类似的球体和其潜在的应用，量化，在细胞间的相互作用的差异。

Tags

Hanging Drop Cell Culture 3D Spheroids Cell-cell Cohesion Cell-substratum Adhesion Monolayer Culture Rigid Substrates Intimate Connections Extracellular Matrix Components Chemical Milieu Physical Forces Cellular Morphology Signaling Collagen Gels Biomaterial Scaffolds Direct Cell-cell Adhesion Architecture Culture Systems ECM Products Physiological Conditions Biological Agent

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