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Measurement of Aggregate Cohesion by Tissue Surface Tensiometry
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Journal JoVE Bio-ingénierie
Measurement of Aggregate Cohesion by Tissue Surface Tensiometry

Measurement of Aggregate Cohesion by Tissue Surface Tensiometry

DOI:
10.3791/2739-v

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12:49 min

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April 08, 2011

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,
1Department of Surgery,UMDNJ-Robert Wood Johnson Medical School

Chapitres

  • 00:05Titre
  • 01:48Aggregate Formation by the Cell Sheet Method
  • 05:36Preparing the Tissue Surface Tensiometer
  • 07:34Aggregate Compression
  • 10:10Tissue Surface Tension Results for Aggregates of Rat Prostate Fibroblasts (RPF) and Rate Prostate Smooth Muscle Cells (RPSMC)
  • 12:10Conclusion

Summary

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We describe a method of measuring binding energy, expressible as tissue surface tension, between cells within 3D tissue-like aggregates. Differences in tissue surface tension have been demonstrated to correlate with invasiveness of lung, muscle, and brain tumors, and are fundamental determinants of establishing spatial relationships between different cell types.

Tags

MeasurementAggregate CohesionIntercellular Binding EnergyThermodynamic PrinciplesEquilibriumBiophysical ConceptsShaking CultureClustersSpheresLiquid SystemsCompressing Spherical AggregatesSurface TensionEmbryonic CellsBiomaterialsCadherin-based Intercellular Cohesion

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