Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / A Protocol for Collecting and Staining Hemocytes…
JoVE Journal
Immunology and Infection

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Immunology and Infection
A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
 
Click here for the English version

A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti

Article DOI: 10.3791/2772-v • 08:33 min • May 16th, 2011
May 16th, 2011 •
1, 1
1Department of Biology, University of Richmond

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

A simplified yet accurate method to collect and stain mosquito hemocytes is described. Our method combines the simplicity of perfusion with the accuracy of high injection techniques to isolate clean preparations of hemocytes in Aedes mosquitoes. This method facilitates studies requiring knowledge of the types of hemocytes and their abundance.

Tags

Protocol Collecting Staining Hemocytes Yellow Fever Mosquito Aedes Aegypti Vectors Disease-causing Pathogens Yellow Fever Virus Malaria Parasites Filarial Worms Innate Immune System Defense Lines Epithelium-lined Mosquito Midgut Hemolymph Circulating Hemocytes Encapsulate And Engulf Pathogens Hematopoietic Tissues Adult Emergence Decline With Age Collect Hemocytes Medically Important Insects Small Size Mosquitoes Limited Volume Of Hemolymph Expulsion Method Cut Proboscis Volume Displacement Method Saline Injection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter