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Chapitres
- 00:05Titre
- 02:00Transfection of Primary Cultured Cortical Neurons
- 03:14Treatment of Primary Cultured Cortical Neurons Grown on 18 mm Coverslips
- 03:46Fixation and Immunocytohistochemistry
- 05:21Quantitative Analysis of Spine Morphologies
- 06:58Time-lapse Imaging
- 09:43Representative Results: Analysis of Dendritic Spine Morphology
- 11:04Conclusion
Numerous recent studies have identified mutations in synaptic proteins associated with brain pathologies. Primary cultured cortical neurons offer great flexibility in examining the effects of these disease-associated proteins on dendritic spine morphology and motility.
Tags
Dendritic SpinesMorphologyCultured NeuronsExcitatory ConnectionsSynapsesActinHebbian PlasticityNeuromodulatory SignalsNeural CircuitsInformation ProcessingSynaptic ProteinsDisease StatesSchizophreniaAutism Spectrum DisordersAberrant Spine PlasticityPathophysiologyCultured Cortical NeuronsHigh-resolution ImagingFixed CellsTime-lapse Imaging
