Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Jessica M. Skeie; Stephen H. Tsang; Vinit B. Mahajan

doi:10.3791/2795

Journal

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Biologie

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Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

Journal JoVE
Biologie
Author Produced

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Journal JoVE Biologie

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

DOI:

10.3791/2795-v

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02:39 min

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April 03, 2011

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Jessica M. Skeie², Stephen H. Tsang, Vinit B. Mahajan²

¹Omics Laboratory,University of Iowa, ²Ophthalmology and Visual Sciences,University of Iowa, ³Harkness Eye Institute,Columbia University College of Physicians and Surgeons

Chapitres

00:01Titre
00:15Anterior Segment Dissection
00:38Lens and Vitreous Evisceration
01:12Retina Evisceration
01:31Centrifugation

Summary

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The dissection technique illustrates evisceration of the vitreous, retina, and lens from the mouse eye, separation by centrifugation, and characterization with protein assays.

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

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Chapitres

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