Whole Mount <em>in Situ</em> Hybridization of E8.5 to E11.5 Mouse Embryos

00:05Titre
01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
03:58Collection of Mouse Embryos
05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
12:38Examples of Whole-mount in situ Hybridizations
13:29Conclusion

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This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

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