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Chapitres
- 00:05Titre
- 01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
- 03:58Collection of Mouse Embryos
- 05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
- 10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
- 12:38Examples of Whole-mount in situ Hybridizations
- 13:29Conclusion
Tags
Whole MountIn Situ HybridizationGene Expression PatternsEmbryosProbe LabelingQuality ControlDIG-labeled RNA ProbesIn Vitro TranscriptionPCRSensitivityEndogenous MRNAFixationStorageProteinase K DigestionHybridization ConditionsPost-hybridization WashesRNase TreatmentNon-specific Probe HybridizationAP-conjugated Antibody
