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JoVE Journal Neuroscience
Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
 

Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury

Article DOI: 10.3791/2803-v • 09:41 min • July 20th, 2011
July 20th, 2011 •
*1,2, *1, 1
1Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research, University of Tuebingen, 2Graduate School for Cellular and Molecular Neuroscience, University of Tuebingen
* These authors contributed equally

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résumé
Automatic Translation

We present a method for chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury. The approach can be used to identify specific transcription factor binding sites and epigenetic modification of histone and DNA important for the regeneration of injured axons in both the peripheral and central nervous system.

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Chromatin Immunoprecipitation Dorsal Root Ganglia Axonal Injury Central Nervous System Peripheral Nervous System Neurite Outgrowth Axonal Regeneration Transcriptional Programs Gene Regulation Injury Model System Transcriptional Activity Animal Removal Nerve Fibers Sciatic Nerve Spinal Cord Transcription Factor Binding Histone Modification DNA Modification ChIP Protocol
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