High-throughput Yeast Plasmid Overexpression Screen

Michael S. Fleming; Aaron D. Gitler

doi:10.3791/2836

Journal

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Biologie

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High-throughput Yeast Plasmid Overexpression Screen

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Biologie

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Journal JoVE Biologie

High-throughput Yeast Plasmid Overexpression Screen

DOI:

10.3791/2836-v

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08:57 min

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July 27, 2011

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Michael S. Fleming, Aaron D. Gitler

¹Neuroscience Graduate Group,University of Pennsylvania School of Medicine , ²Department of Cell and Developmental Biology,University of Pennsylvania School of Medicine

Chapitres

00:05Titre
00:56Preparations for Yeast Transformation
02:19Yeast Transformation Using a Liquid Handling Robot
06:04Spotting Assay
07:10Representative Results: Plasmid Overexpression Screen
08:43Conclusion

Summary

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Here we describe a plasmid overexpression screen in Saccharomyces cerevisiae, using an arrayed plasmid library and a high-throughput yeast transformation protocol with a liquid handling robot.

High-throughput Yeast Plasmid Overexpression Screen

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Chapitres

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