Journal
/
Biologie
/
Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Preparation, Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

DOI:
10.3791/2844-v

•

13:21 min

•

July 21, 2011

•

, , , ,
1Department of Chemistry,Wayne State University

Chapitres

  • 00:05Titre
  • 01:20Metalation Using LnCl3 Salts
  • 03:33Dialysis Workup
  • 07:00Assessment of the Presence of Free Metal and Determination of Water-coordination Number (q)
  • 09:50Relaxation Measurements
  • 11:35Representative Results of Characterization
  • 12:48Conclusion

Summary

Traduction automatique

Traduction automatique

We demonstrate the metalation, purification, and characterization of lanthanide complexes. The complexes described here can be conjugated to macromolecules to enable tracking of these molecules using magnetic resonance imaging.

Tags

PreparationPurificationCharacterizationLanthanide ComplexesContrast AgentsMagnetic Resonance ImagingPolyaminopolycarboxylate-based LigandsChelateFunctional GroupsConjugationAmine-reactive Activated EstersIsothiocyanate GroupsThiol-reactive MaleimidesMetalation ProceduresMetal Starting MaterialsPurification MethodsBiomedical Scientists

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2844?article_type=v

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
check_url/fr/2844?article_type=v

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging
check_url/fr/2844?article_type=v

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
check_url/fr/2844?article_type=v

Phase Contrast and Differential Interference Contrast (DIC) Microscopy
check_url/fr/2844?article_type=v

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
check_url/fr/2844?article_type=v

Microinjection of Medaka Embryos for use as a Model Genetic Organism
check_url/fr/2844?article_type=v

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
check_url/fr/2844?article_type=v

Quantifying Mixing using Magnetic Resonance Imaging
check_url/fr/2844?article_type=v

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
check_url/fr/2844?article_type=v

FRET Imaging in Three-dimensional Hydrogels

Read Article