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Bacterial Immobilization for Imaging by Atomic Force Microscopy
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Journal JoVE Bio-ingénierie
Bacterial Immobilization for Imaging by Atomic Force Microscopy

Bacterial Immobilization for Imaging by Atomic Force Microscopy

DOI:
10.3791/2880-v

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10:03 min

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August 10, 2011

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, , , ,
1Biological and Nanoscale Systems Group, Biosciences Division,Oak Ridge National Laboratory, 2Department of Biochemistry and Cellular and Molecular Biology,University of Tennessee , 3Department of Surgery,Eastern Virginia Medical School, 4Center for Nanophase Materials Sciences Division,Oak Ridge National Laboratory

Chapitres

  • 00:05Titre
  • 02:07Preparation of Gelatin Coated Mica
  • 04:13Mounting Bacteria on Gelatin Coated Mica
  • 06:48Atomic Force Microscopy
  • 08:11Representative Results
  • 08:55Conclusion

Summary

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Traduction automatique

Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).

Tags

Bacterial ImmobilizationAtomic Force MicroscopyHigh-resolution ImagingImaging CellsBiomoleculesLiquid EnvironmentScanning AFM Cantilever TipSample ImmobilizationMinimally Invasive ImmobilizationPorcine Gelatin CoatingNegative BacteriaElectrostatic InteractionFactors Affecting ImmobilizationMicrobial Cell Imaging

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