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Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy
Journal JoVE
Biologie
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Journal JoVE Biologie
Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

Micro-Mechanical Characterization of Lung Tissue Using Atomic Force Microscopy

DOI:
10.3791/2911-v

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11:10 min

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August 28, 2011

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1Molecular and Integrative Physiological Sciences, Department of Environmental Health,Harvard School of Public Health

Chapitres

  • 00:05Titre
  • 01:50Preparation of Lung Tissue Strips
  • 03:11AFM Microindentation and Fluorescent Imaging
  • 07:14AFM Data Extraction
  • 08:48Representative AFM Results
  • 10:48Conclusion

Summary

Traduction automatique

Traduction automatique

The stiffness of the extracellular matrix strongly influences multiple behaviors of adherent cells. Matrix stiffness varies spatially throughout a tissue, and undergoes modification in various disease conditions. Here we develop methods to characterize spatial variations in stiffness in normal and fibrotic mouse lung tissue using atomic force microscopy microindentation.

Tags

Micro-Mechanical CharacterizationAtomic Force MicroscopyMatrix StiffnessAdherent CellsMacro ScaleSpatial VariationDisease ProcessesMatrix RemodelingCellular PhysiologyHealth And DiseaseLung ParenchymaAFM Microindentation

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