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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
DOI:

13:15 min

June 01, 2011

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Chapitres

  • 00:05Titre
  • 01:28Preparation of Chemically Modified Probe DNA
  • 02:48Sensor Preparation
  • 05:30Sensor Testing, DNA Detection
  • 07:57Sensor Testing, Antibody Detection
  • 09:47Sensor Testing, Small Molecule Detection
  • 11:24Representative Results obtained using Electrochemical-DNA Biosensors
  • 12:27Conclusion

Summary

Traduction automatique

"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.

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