Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:33Growing Cotton Seedlings
- 02:43Constructing the VIGS Vector Carrying the GrCla1 Gene
- 04:55Virus Induced Gene Silencing Inoculation
- 08:40Examples of Albino Phenotypes on VIGS Silenced Cotton Plants
- 09:26Conclusion
We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.
Tags
Agrobacterium-mediatedVirus-Induced Gene Silencing AssayCottonGossypium HirsutumMolecular BreedingGene Functional AnalysisGenome SizeGene DuplicationPolyploidyGrowth CycleGenetic TransformationHigh ThroughputFunctional Genetic/genomic StudyVIGSPost-Transcriptional Gene Silencing (PTGS)Viral ProliferationAgrobacterium-mediated VIGSDicots SpeciesSolanaceaeArabidopsisLegume SpeciesMonocots SpeciesBarleyWheatMaizeFunctional Genomic StudiesTobacco Rattle Virus (TRV)
