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Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
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Chapitres
- 00:05Titre
- 02:08Capillary and Micro-Electrode Preparation
- 03:48Plasmid DNA Preparation
- 04:47Surgery, Injection and Electroporation
- 07:03In utero Cortical and Deep-tissue Plasmid Electroporation
- 08:34Conclusion
A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development.
Tags
MouseIn Utero ElectroporationGene TransfectionDeveloping BrainGene TransferKnock-outKnock-inSpecific CRE LineTransfection MethodCandidate GenesTransgenic AnimalsBrain AreasEmbryonic LethalityElectrodesOptic Fiber Optic Light CableDNA Solution InjectionVentriclesNeedle Type ElectrodesBrain Area Patterning
