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The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse
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The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

DOI:
10.3791/3039-v

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08:27 min

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July 25, 2011

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, ,
1Department of Pharmacology and Physiology,University of Rochester, 2Department of Neurology,University of Alabama at Birmingham, 3Departments of Anesthesiology, Pharmacology and Physiology,University of Rochester

Chapitres

  • 00:05Titre
  • 01:22Pre-surgical Preparation
  • 03:25Surgical Procedure and MCA Ligation
  • 05:29TTC Staining and Determination of Stroke Colume
  • 06:06Directions to use ImageJ Software
  • 07:26Representative Results
  • 08:07Conclusion

Summary

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Middle cerebral artery (MCA) ligation is a technique to study focal cerebral ischemia in animal models. In this method, the middle cerebral artery is exposed by craniotomy and ligated by cauterization. This method gives highly reproducible infarct volumes and increased post-operative survival rates compared to other methods available.

Tags

Permanent MCA LigationFocal Cerebral IschemiaStrokeAnimal ModelsPMCAoCraniotomyElectrocoagulationInfarctsCortical RegionsStriatal RegionsNeuroprotective CompoundsReproducible InfarctsPost-survival RatesMCA Artery

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