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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Chapitres
- 00:05Titre
- 01:08Preparation of Listeria Inoculum and Intravenous Injection of Mice
- 04:13Removal of Liver and Spleen from Listeria-infected Mice for Analysis
- 05:57Preparation of Hepatic Cell Suspensions for FACS Analysis
- 08:40Measurement of Bacterial Load in Tissues from Listeria-infected Mice
- 10:26Representative Data for Bacterial Load and Immune Cell Analysis
- 12:24Conclusion
Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.
Tags
Bacterial LoadImmune ResponsesMiceListeria MonocytogenesGram-positiveIntracellular PathogenSystemic InfectionSpleenLiverCD8α+ Dendritic CellsKupffer CellsPhagocytosedBacterial ProteinsPhagosomeCytosolInnate Immune CellsMonocytesNeutrophilsNK CellsDendritic CellsBactericidal MechanismsCD8+ T CellsClearance Of ListeriaHost Immune ResponsesInbred Mouse StrainsGenetic Studies
