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Immunohistochemistry: पैराफिन धारा वेक्टर लैब्स से Vectastain एबीसी किट का उपयोग
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
DOI:

21:38 min

October 01, 2007

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Chapitres

  • 00:02Titre
  • 00:06Introduction
  • 00:49Dewaxing and Rehydrating Paraffin Sections
  • 03:20Unmasking Antigen with Heat-Induced Antigen Retrieval
  • 05:14Blocking Endogenous Peroxidase Activity and Nonspecific Binding of
  • 08:17Adding Primary and Secondary Antibodies
  • 11:39Preparing and Incubating Slides in the ABC Reagent
  • 13:21Preparing and Reacting Slides with DAB Substrate Reagent
  • 16:10Counterstaining and Mounting the Slides
  • 20:46Visualizing Anti-Tyrosine Hydroxylase Slides
  • 21:07Conclusion

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