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Chapitres
- 00:05Titre
- 01:25Extracting Collagen from Rat Tails
- 02:513-Dimensional Assay Creation
- 05:14Antibody Staining in Preparation for Optical Projection Tomography
- 07:07Optical Projection Tomography, Reconstruction, and 3D Quantification
- 09:27Representative 3D Constructions
- 10:00Conclusion
Nós desenvolvemos um à base de colágeno<em> In vitro</em> Ensaio que promove a proliferação e invasão a partir de amostras de todos os subtipos de câncer de mama. Tomografia projeção óptica, uma técnica de microscopia tridimensional foi utilizado para visualizar e quantificar a expansão do tumor. Este ensaio poderá ser utilizada para quantificar a resposta à droga das amostras de tumor individual.
Tags
Long-term CultureHuman Breast Cancer SpecimensOptical Projection TomographyBreast CancerMortalityWestern WorldSpread Of Breast CancerPatient PrognosisExperimental SystemsCell LinesPrimary TumoursPleural EffusionsSub-types Of Breast CancersPoor Prognosis Luminal B TumoursTumour MicroenvironmentCulture SystemRat Collagen I CushionsHistologyAdipocytesEpithelial CellsFibroblastsMalignant Epithelial CellsSquamoid MorphologyNuclear PleomorphismMitotic FiguresApoptotic BodiesOptical Projection Tomography (OPT)3D Imaging Technology
