Journal
/
Biologie
/
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

DOI:
10.3791/3087-v

•

16:27 min

•

September 14, 2011

•

, ,
1Brigham and Women’s Hospital / Harvard Medical School,Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering,Cornell University

Chapitres

  • 00:05Titre
  • 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
  • 03:30Substrate Strain Experiments
  • 06:50Substrate Strain Analysis
  • 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
  • 09:57Microneedle Manipulation Experiment
  • 12:19Microneedle Manipulation Analysis
  • 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
  • 15:58Conclusion

Summary

Traduction automatique

Traduction automatique

We present two independent, microscope-based tools to measure the induced nuclear and cytoskeletal deformations in single, living adherent cells in response to global or localized strain application. These techniques are used to determine nuclear stiffness (i.e., deformability) and to probe intracellular force transmission between the nucleus and the cytoskeleton.

Tags

Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3087?article_type=v

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
check_url/fr/3087?article_type=v

Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
check_url/fr/3087?article_type=v

Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells
check_url/fr/3087?article_type=v

Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica
check_url/fr/3087?article_type=v

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
check_url/fr/3087?article_type=v

Super-resolution Imaging of the Bacterial Division Machinery
check_url/fr/3087?article_type=v

Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
check_url/fr/3087?article_type=v

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
check_url/fr/3087?article_type=v

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
check_url/fr/3087?article_type=v

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Read Article