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生物物理检测探头相间细胞核的力学性能：基材应变的应用和微针操纵
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Biologie
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Journal JoVE Biologie
Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation
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生物物理检测探头相间细胞核的力学性能：基材应变的应用和微针操纵

DOI:
10.3791/3087-v

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16:27 min

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September 14, 2011

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, ,
1Brigham and Women’s Hospital / Harvard Medical School,Department of Medicine, Cardiovascular Division, 2Weill Institute for Cell and Molecular Biology & Department of Biomedical Engineering,Cornell University

Chapitres

  • 00:05Titre
  • 01:47Preparation of Silicone Membrane Dishes and Adherence of Cells
  • 03:30Substrate Strain Experiments
  • 06:50Substrate Strain Analysis
  • 08:35Representative Results for Substrate Strain Application on the Interphase Cell Nucleus
  • 09:57Microneedle Manipulation Experiment
  • 12:19Microneedle Manipulation Analysis
  • 13:50Representative Results for Microneedle Manipulation of the Interphase Cell Nucleus
  • 15:58Conclusion

Summary

Traduction automatique

Traduction automatique

我们现在两个独立的显微镜为基础的工具来衡量，在应对全球性或局部应变的应用单一，生活贴壁细胞诱导核和细胞骨架变形。这些技术被用于确定核刚度（即变形）和探测细胞内的细胞核和细胞骨架的之间的传力。

Tags

Biophysical AssaysMechanical PropertiesInterphase Cell NucleusSubstrate Strain ApplicationMicroneedle ManipulationEukaryotic CellsNucleus StiffnessCytoskeletonBiomechanical BehaviorPhysiological ConditionsPathological ConditionsMigrating NeutrophilsInvading Cancer CellsExtravasationNarrow SpacesMuscleStructural SupportRepetitive Mechanical StressCellular ArchitectureNuclear Envelope ProteinsLaminsNesprinsNucleo-cytoskeletal CouplingHuman Diseases

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