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Immunologie et infection
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Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

DOI:
10.3791/3094-v

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12:39 min

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June 24, 2011

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, , ,
1Department of Laboratory Medicine and Pathology,Mayo Clinic

Chapitres

  • 00:05Titre
  • 01:47Experimental Preparation and Biosafety Procedures
  • 03:13Preparation of the Inoculum
  • 05:05Plating the Inoculum
  • 08:07Determining Antibiotic Resistance Manually
  • 09:12Determining Antibiotic Resistance Using the Vizion Platform
  • 10:10Determining Sample Purity and Performing Colony Counts
  • 10:57Representative Antimicrobial Susceptibility Assay
  • 12:00Conclusion

Summary

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Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.

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Antimicrobial Susceptibility TestingMycobacterium Tuberculosis ComplexFirst Line DrugsSecond Line DrugsBroth DilutionMicrotiter Plate FormatAgar Method Of ProportionMacrobroth TestingBACTECVersaTREKBacT/ALERTAgar Proportion MethodResistance CalculationDrug-containing AgarDrug-free AgarMacrobroth MethodsCritical Drug ConcentrationsMYCOTBTuberculosis Treatment

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