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测绘激光扫描Photostimulation抑制神经回路

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

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测绘激光扫描Photostimulation抑制神经回路

DOI:

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09:50 min

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October 06, 2011

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Taruna Ikrar, Nicholas D. Olivas, Yulin Shi, Xiangmin Xu²

¹Department of Anatomy and Neurobiology, School of Medicine,University of California, Irvine, ²Department of Biomedical Engineering, School of Engineering,University of California, Irvine

Chapitres

00:05Titre
01:32Brain Slice Preparation
02:17Equipment Startup
03:24Whole Cell Patch-clamp Recording
05:52Laser Scanning Photostimulation
07:44Representative Results for Electrophysiological Recording and Photostimulation Mapping

Summary

Traduction automatique

本文介绍了激光扫描photostimulation在表达绿色荧光蛋白在有限的抑制性神经元的人口的转基因小鼠的全细胞记录相结合的方法。该技术可以广泛的测绘和定量分析当地具体的抑制皮层神经元的突触电路。

Tags

Inhibitory Neurons Neuronal Circuits Laser Scanning Photostimulation Cortical Function Subtypes Immunochemical Properties Morphological Properties Physiological Properties Local Circuit Connections Excitatory Synaptic Input Inhibitory Synaptic Input Laser Scanning Photostimulation (LSPS)Electrical Stimulation Glutamate Puff Stimulation Mapping Resolution Cortical Circuit Analysis Whole-cell Patch Clamping

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