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Mapeamento Inibitória Circuitos Neuronal por Laser Scanning fotoestimulação
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
DOI:

09:50 min

October 06, 2011

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Chapitres

  • 00:05Titre
  • 01:32Brain Slice Preparation
  • 02:17Equipment Startup
  • 03:24Whole Cell Patch-clamp Recording
  • 05:52Laser Scanning Photostimulation
  • 07:44Representative Results for Electrophysiological Recording and Photostimulation Mapping

Summary

Traduction automatique

This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.

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