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Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography
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Médecine
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Journal JoVE Médecine
Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

Mesenteric Artery Contraction and Relaxation Studies Using Automated Wire Myography

DOI:
10.3791/3119-v

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07:51 min

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September 22, 2011

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, , ,
1Julius L. Chambers Biomedical/Biotechnology Research Institute,North Carolina Central University, Durham, 2Department of Biology,North Carolina Central University, Durham, 3Department of Physiology & Pharmacology and Hypertension & Vascular Research Center,Wake Forest University School of Medicine

Chapitres

  • 00:05Titre
  • 01:43Isolation of Rat Mesenteric Small Artery
  • 02:45Mounting of Vessel
  • 04:43Normalization
  • 05:31Measurement of Responses
  • 06:24Contraction and Relaxation in Mesenteric Arteries
  • 07:15Conclusion

Summary

Traduction automatique

Traduction automatique

An automated myography method for force measurements in isolated mesenteric arteries is described. It employs a Mulvany-Halpern Auto Dual Wire Myograph 510A to determine responses to phenylephrine and extracellular calcium. The method allows consistent determination of isometric responses to agonists in small vessels of diameters of 60 - 300 μm, independently.

Tags

Mesenteric ArteryContractionRelaxationAutomated Wire MyographyProximal Resistance VesselsBlood FlowRat Mesenteric ArteryMyography TechniqueIsometric ConditionsForce ProductionSensitivityAgonistsStretchCompliance Of Mounting WiresStainless Steel WiresTungsten WiresOxidationRecorded ResponsesVascular Smooth Muscle Cell ReceptorsPhenylephrineExtracellular Calcium (Ca2+e)

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