Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Temple University,Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service,Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy,Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology,Temple University School of Medicine
Chapitres
- 00:05Titre
- 01:19Preparation for Surgery
- 02:19Laminectomy and Surgical Exposure of the L5 Dorsal Root
- 03:21Rhizotomy/Dorsal Root Crush
- 04:10Image Acquisition and Post-op Procedures
- 04:51Repeated Imaging
- 05:32Axonal Regeneration Following Dorsal Root Crush
- 06:16Conclusion
Een<em> In vivo</em> Imaging-protocol om de primaire sensorische axonen volgende achterwortelganglia verpletteren monitor is beschreven. De procedures maken gebruik van wide-field fluorescentie microscopie en thy1-YFP transgene muizen, en staan herhaalde beeldvorming van regeneratie van axonen meer dan 4 cm in de PNS en axon interacties met de interface van het CZS.
Tags
Live ImagingDorsal Root AxonsRhizotomySpinal Root InjuriesChronic PainSensory LossRegenerationDorsal Root Entry Zone (DREZ)Molecular EventsCellular EventsNerve InjuryPostmortem AnalysesAxon RegenerationReal-time EventsLiving AnimalNeurons Serially In VivoOpticsMouse TransgenicsThy1-GFP Transgenic MiceFluorescent ColorsIn Vivo ImagingMuscleBrainPhysiological MechanismsStatic AnalysesSpinal Cord Imaging
