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Increasing cDNA Yields from Single-cell Quantities of mRNA in Standard Laboratory Reverse Transcriptase Reactions using Acoustic Microstreaming
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Chapitres
- 00:05Titre
- 01:43Micromixing an RT Reaction
- 02:59The Micromixing Advantage in RT
- 04:10Conclusion
We describe a novel method for increasing cDNA yield from single-cell quantities of mRNA in otherwise standard laboratory reverse transcription reactions. The novelty resides in the use of a micromixer, which utilizes the phenomenon of acoustic microstreaming, to mix fluids at microliter scales more effectively than shaking, vortexing or trituration.
Tags
CDNA YieldsSingle-cell QuantitiesMRNALaboratory Reverse Transcriptase ReactionsAcoustic MicrostreamingGene ExpressionSingle-cell LevelLabile MRNADegradationStable CDNA CopyEfficiencyStandard ReagentsMicroliter VolumesMixing ReagentsMicro-scale Mixing TechniquesMicrofluidic-based Mixing TechnologiesRT Reaction Yields
