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Kryokonservering av Mouse Embryos ved etylenglykol-Based forglassing
Journal JoVE
Biologie
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Journal JoVE Biologie
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification

Kryokonservering av Mouse Embryos ved etylenglykol-Based forglassing

DOI:
10.3791/3155-v

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06:00 min

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November 18, 2011

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, , , ,
1RIKEN BioResource Center

Chapitres

  • 00:05Titre
  • 01:13Vitrification of 2-cell Mouse Embryos
  • 02:22Thawing Vitrified 2-cell Mouse Embryos
  • 05:03Representative Results
  • 05:30Conclusion

Summary

Traduction automatique

Traduction automatique

En etylenglykol-basert forglassing metode for mus embryo er beskrevet. Det er en fordel til andre metoder i sin enkelhet og lave embryonale toksisitet, og derfor kan grovt anvendelig til mange stammer av mus, inkludert innavlet og genmodifiserte mus.

Tags

CryopreservationMouse EmbryosEthylene Glycol-based VitrificationBiomedical SciencesGenetic ModificationsSlow Freezing MethodsVitrification MethodsCryoprotectantsToxicityEmbryo DevelopmentRIKEN BioResource CenterMouse StrainsGenetic BackgroundsPhenotypesVitrification TechniqueEmbryo SurvivalCryopreservation SolutionEthylene GlycolDMSOFicollSucrose

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