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Chapitres
- 00:05Titre
- 01:49Lung Isolation
- 04:05Preparation of a Single Cell Suspension from Lung Tissue
- 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
- 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
- 14:49Representative Analysis of Isolated MSCs
- 16:08Conclusion
Tags
IsolationCharacterizationHoechstlow CD45negativeMouse LungMesenchymal Stem CellsRegulatorsRegenerationFibrosisInflammationAngiogenesisTumor FormationInjuryDiseasesPulmonary FibrosisArterial HypertensionTechnologyDefine Set Of MarkersRepeated IsolationWell-characterized Stem Cell PopulationFlow CytometrySpecific Cell TypeFunction
