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Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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Biologie
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Journal JoVE Biologie
Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy

DOI:
10.3791/3163-v

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11:47 min

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April 07, 2012

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, , , , ,
1Biosphere Oriented Biology Research Unit,RIKEN Advanced Science Institute, 2Graduate School of Nanobioscience, Yokohama City University, 3Advanced NMR Metabomics Research Team,RIKEN Plant Science Center, 4Graduate School of Bioagricultural Science,Nagoya University

Chapitres

  • 00:02Titre
  • 01:28Preparation of filters to remove extractables and filtration of sample for metabolite concentration
  • 04:44Extraction of aqueous-soluble metabolites
  • 07:48NMR spectroscopy and data analysis
  • 11:15Conclusions

Summary

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A method for metabolite extraction from microbial planktonic communities is presented. Whole community sampling is achieved by filtration onto specially prepared filters. After lyophilization, aqueous-soluble metabolites are extracted. This approach allows for application of environmental metabolomics to trans-omics investigations of natural or experimental microbial communities.

Tags

ConcentrationMetabolitesLow-densityPlanktonic CommunitiesEnvironmental MetabolomicsNuclear Magnetic Resonance SpectroscopyNMR SpectroscopyGas Chromatography-mass SpectrometryGC-MSUntargeted AnalysesStructural InformationQuantitative AnalysisStatistical AnalysisMetabolite Spectra DatabasesOmics LevelsTranscriptomicsGenomicsPhysiological ResponsesTaxaSensitivityNatural Microbial Systems

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