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Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement
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Chapitres
- 00:05Titre
- 01:08Preparation of the Patterned Master
- 02:30Preparation of PDMS Stamp
- 03:11Preparation of the Patterned Gold Substrate
- 05:08Applying Protein and Cells to the Patterned Substrate
- 06:12Results of Creating Two-Dimentional Patterned Substrates
- 08:06Conclusion
Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.
