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Quantifying Agonist Activity at G Protein-coupled Receptors
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Journal JoVE Biologie
Quantifying Agonist Activity at G Protein-coupled Receptors

Quantifying Agonist Activity at G Protein-coupled Receptors

DOI:
10.3791/3179-v

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11:45 min

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December 26, 2011

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, ,
1Department of Pharmacology,University of California, Irvine, 2Department of Pharmacology,University of California, 3Schmid College of Science,Chapman University

Chapitres

  • 00:05Titre

Summary

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A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.

Tags

QuantifyingAgonist ActivityG Protein-coupled ReceptorsSignaling PathwayCell ResponseSingle Receptor AnalysisDownstream ResponseAgonist Affinity ConstantActive StateInactive StateLigandsReceptor ActivationConcentration Of AgonistObserved Affinity ConstantEfficacyMethods For Analysis

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