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Immunology and Infection

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JoVE Journal Immunology and Infection
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
 

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate

Article DOI: 10.3791/3182-v • 13:09 min • September 9th, 2011
September 9th, 2011 •
1,2, 1,2, 1,2, 1,2, 1,2, 1, 1
1Department of Immunotherapeutics (Medinet), University of Tokyo Hospital, 2MEDINET Co., Ltd

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résumé
Automatic Translation

A method to expand γδ T cells from peripheral blood mononuclear cells (PBMC) is described. PBMC-derived γδ T cells are stimulated and expanded using zoledronate and interleukin-2 (IL-2). Large scale expansion of γδ T cells can be applied to autologous cellular immunotherapy of cancer.

Tags

Human Peripheral Blood γδ T Cells Expansion Zoledronate Stress-induced Antigens Anti-tumor Activity Anti-infective Activity Vγ9Vδ2 T Cell Receptor Major Histocompatibility Complex-independent Cytolytic Functions Th1-like Effector Functions Cancer Immunotherapy Phosphoantigens (E)-4-hydroxy-3-methyl-but-2-enyl Pyrophosphate (HMBPP) Isoprenoid Biosynthesis Isopentenyl Pyrophosphate (IPP) Mevalonate Pathway Dysregulation Tumor Cells Aminobisphosphonates Pamidronate Farnesyl Pyrophosphate Synthase (FPPS) Intracellular Levels Of IPP
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