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Undersøgelse af Actin cytoskeleton i Live endoteliale celler, der udtrykker GFP-Actin
Journal JoVE
Biologie
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Journal JoVE Biologie
Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin

Undersøgelse af Actin cytoskeleton i Live endoteliale celler, der udtrykker GFP-Actin

DOI:
10.3791/3187-v

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08:37 min

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November 18, 2011

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,
1Department of Physiology,Louisiana State University Health Sciences Center

Chapitres

  • 00:05Titre
  • 01:27Transfection of HUVEC with GFP-Actin
  • 03:25Set up: Live-cell Imaging Chamber and Stage Heater
  • 05:20Live-cell Imaging Microscope Data Acquisition and Analysis
  • 06:14Kymograph Analysis of HUVEC
  • 08:08Conclusion

Summary

Traduction automatique

Traduction automatique

Mikroskopisk billedbehandling af levende endothelceller udtrykker GFP-aktin tillader karakterisering af dynamiske ændringer i cytoskeletal strukturer. I modsætning til teknikker, der anvender faste prøver, giver denne metode en detaljeret vurdering af tidsmæssige ændringer i actin cytoskeleton i de samme celler før, under og efter forskellige fysiske, farmakologiske eller inflammatoriske stimuli.

Tags

Actin CytoskeletonEndothelial CellsGFP-ActinPermeabilityAdhesive JunctionsJunctional IntegrityCortical Actin BeltActin Stress FibersCentripetal TensionInflammatory MediatorsEndothelial PermeabilityLive-cell ImagingDynamic Nature Of Actin Structures

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