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Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
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Neurosciences
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Journal JoVE Neurosciences
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

DOI:
10.3791/3195-v

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11:35 min

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October 29, 2011

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, ,
1SISSA,International School for Advanced Studies, 2Istituto di Biofisica,Consiglio Nazionale delle Ricerche, 3SISSA Unit,Italian Institute of Technology

Chapitres

  • 00:05Titre
  • 00:08Introduction
  • 01:35Instrumentation
  • 03:37Dissociation of Olfactory Sensory Neurons
  • 07:51Recording
  • 09:37Representative Results
  • 11:09Conclusion

Summary

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Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds. Here, we show how to obtain patch-clamp recordings combined with photolysis of caged cAMP or caged Ca for the study of olfactory transduction in dissociated mouse olfactory sensory neurons.

Tags

Flash PhotolysisCaged CompoundsOlfactory Sensory NeuronsPatch-clamp RecordingsPhotolysis Of Caged CompoundsOlfactory TransductionDissociated Mouse Olfactory Sensory NeuronsCiliaOdorant BindingReceptorsCAMPCyclic Nucleotide-gated (CNG) ChannelsCa EntryCa-activated Cl ChannelsMouse Olfactory EpitheliumFlash LampUltraviolet FlashesWhole-cell Voltage-clamp Configuration

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