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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay
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Journal JoVE Biologie
Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

DOI:
10.3791/3202-v

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10:28 min

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January 08, 2012

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, ,
1Obstetrics, Gynecology and Women’s Health,University of Missouri

Chapitres

  • 00:05Titre
  • 01:30Dissection of the Mouse Placenta
  • 03:29Isolation of Trophoblast Cells
  • 06:53Trophoblast Invasion Assay
  • 08:21Representative Results

Summary

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In this protocol, we describe the dissection of placentae from the mouse on pregnancy d10.5, followed by isolation of trophoblast cells using a Percoll gradient. We then demonstrate use of the isolated cells in a matrigel invasion assay.

Tags

IsolationPrimary Mouse Trophoblast CellsTrophoblast Invasion AssayPlacentaNutrientsGassesGrowth FactorsWastesPlacental DevelopmentFetusMotherEmbryonic LethalityCell TypeFetal PortionToolNormal Placental DevelopmentAbnormal Placental DevelopmentCell LinesSpecific Stages Of PregnancyPrimary CulturesTransgenic MiceParticular GenesPercoll GradientTrophoblast Cell PopulationMouse PlacentasHuman Trophoblast Cell IsolationPurity AssessmentImmunocytochemical StainingCytokeratin 74Matrigel Invasion AssayTrophoblast Invasiveness In Vitro

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