Journal
/
Médecine
/
Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR
Journal JoVE
Médecine
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR

Detection and Genogrouping of Noroviruses from Children’s Stools By Taqman One-step RT-PCR

DOI:
10.3791/3232-v

•

15:16 min

•

July 22, 2012

•

, , , , , , , , ,
1Laboratorio de Investigación y Desarrollo (LID),Universidad Peruana Cayetano Heredia, 2Bloomberg School of Public Health,Johns Hopkins University, 3Laboratorio de Diagnostico Molecular, Facultad de Medicina,University of Concepcion,Chile, 4University of California San Diego School of Medicine

Chapitres

  • 00:05Titre
  • 01:37In-house Silica-guanidinium Method
  • 02:02Preparation of Silica Suspension for RNA Extraction
  • 03:24Extraction with Guanidine & Silica
  • 06:08Commercial Extraction of Viral RNA: QIAamp Viral RNA Kit
  • 08:32Detection of NoV in Extracted RNA by One-step Real Time PCR with Specific Taqman Probes
  • 14:11Conclusion

Summary

Traduction automatique

Traduction automatique

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

Tags

NorovirusesGenogroupingTaqman One-step RT-PCRAcute GastroenteritisRNA VirusesGenetic DiversityNew StrainsGenogroupsGIGIIGenotypesSubtypesDiagnosisTreatmentRNA ExtractionQIAmp Viral RNA Commercial KitSilica Gel MembraneBuffersMicrospinDiarrheaPathogenesis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/3232?article_type=v

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
check_url/fr/3232?article_type=v

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
check_url/fr/3232?article_type=v

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model
check_url/fr/3232?article_type=v

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
check_url/fr/3232?article_type=v

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
check_url/fr/3232?article_type=v

Pancreatic Duct Infusion: An Effective and Selective Method of Drug and Viral Delivery
check_url/fr/3232?article_type=v

Electrophysiological Measurement of Noxious-evoked Brain Activity in Neonates Using a Flat-tip Probe Coupled to Electroencephalography
check_url/fr/3232?article_type=v

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
check_url/fr/3232?article_type=v

Handheld Metal Detector Screening for Metallic Foreign Body Ingestion in Children
check_url/fr/3232?article_type=v

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Read Article