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In vivo Dual Substrate Bioluminescent Imaging
Journal JoVE
Médecine
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Journal JoVE Médecine
In vivo Dual Substrate Bioluminescent Imaging

In vivo Dual Substrate Bioluminescent Imaging

DOI:
10.3791/3245-v

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07:33 min

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October 11, 2011

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, , ,
1Case Comprehensive Cancer Center,Case Western Reserve University

Chapitres

  • 00:05Titre
  • 01:02Stable Expression of CMV-Driven Renilla Luciferase
  • 01:36Expression, Selection, and Functional Verification of Inducible Promoter-Driven Firefly Luciferase
  • 02:32Establishing 4T1 Primary Mammary Tumors
  • 03:25Initial Dual Luminescent Imaging
  • 04:22Longitudinal Luminescent Imaging
  • 05:17Dual Bioluminescent Imaging of Two Unique Cell Types in a Single Animal
  • 06:16In vivo RLRs from Dual Bioluminescent Imaging
  • 07:10Conclusion

Summary

Traduction automatique

Traduction automatique

Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.

Tags

In VivoDual Substrate Bioluminescent ImagingBreast Cancer CellsPrimary TumorsMetastatic SitesIn Vivo Bioluminescent Imaging TechnologiesTumor GrowthLongitudinal StudyResearchersBreast Cancer MetastasisCell Signaling SystemsGene ExpressionTumor DevelopmentMetastatic ProgressionLuciferase EnzymesFirefly LuciferaseSea Pansy Luciferase

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