Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:06Introduction
- 00:51Cardiac Puncture and Dissection of Thymus
- 02:52Isolation of Antigen Presenting Cells (APCs) from Thymus
- 06:24Activation of OVA-specific T cells by Thymic APCs
- 12:55Adoptive Transfer of OVA-specific T cells
- 19:27Challenging with OVA in the Ear
- 21:02Measurment of Inflammation in the Ear
- 21:27Conclusion
Tags
InductionMonitoringAdoptive Delayed-type HypersensitivityRatsDTHInflammatory ReactionCCR7-Effector Memory T LymphocytesAntigenImmune SystemImmunosuppressantsCutaneous DTHAdoptive TransferActive ImmunizationIntradermal ChallengeOvalbuminTuberculinTetanus ToxoidKeyhole Limpet HemocyaninAutoantigenMyelin Basic Protein (MBP)Experimental Autoimmune Encephalomyelitis (EAE)Lewis RatsStimulationOvalbumin-specific T CellsIntraperitoneally InjectionEquilibrate In VivoPinnaSaline3-72 Hours Later
