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Journal
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Immunologie et infection
/
A Method for Labeling Vasculature in Embryonic Mice
Journal JoVE
Immunologie et infection
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Journal JoVE
Immunologie et infection
A Method for Labeling Vasculature in Embryonic Mice
A Method for Labeling Vasculature in Embryonic Mice
DOI:
10.3791/3267-v
•
09:58 min
•
October 07, 2011
•
Jerrod L. Bryson
,
Mark C. Coles
,
Nancy R. Manley
1
Department of Cellular Biology
,
University of Georgia
,
2
Centre for Immunology and Infection, Department of Biology and HYMS
,
University of York
,
3
Department of Genetics
,
University of Georgia
Chapitres
00:05
Titre
01:06
Labeling Embryonic Vasculature using Fluoroscein-labeled Dextran and GSL I-isolectin B4 Facial Vein Injections and Skin Sample Preparation
03:23
Multi-color Labeling of Thymus Vasculature and Perivaculature Cells for Cryosections
04:53
Multi-color Labeling and Image Acquisition of Thymus Vasculature and Perivascular Cells for Vibratome Sections
08:47
Results: Vascular Labeling
09:26
Conclusion
Summary
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This article describes a method for labeling embryonic skin and thymus blood vessels.
Tags
Labeling Vasculature
Embryonic Mice
Blood Vessel Network
Organogenesis
Thymus
Vasculature Formation
Thymocyte Entry
Mature T-cell Exit
Blood Vessel Defects
FITC-dextran
Griffonia Simplicifolia Lectin I
GSL 1 – Isolectin B4
CD31 Antibody Staining
Thymic Perivascular Mesenchyme
Cryosections
Vibratome Sections
Thymus Vascular Defects
TEC-derived Molecules
Vascular Networks
Multiple Organs
Article
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