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Ice Cap: Um Método para Crescer Arabidopsis Plantas e tomate em placas de 96 poços para High-Throughput Genotipagem
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
DOI:

11:15 min

November 09, 2011

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Chapitres

  • 00:05Titre
  • 02:04Preparation of Ice-Cap Seedling Plates with Agar Growth Media
  • 02:53Sowing and Germination of Seedlings in Ice-Cap Seedling Plates
  • 03:52Transfer of Seedling Plates to the Ice-Cap Fountain
  • 05:46Harvesting of Root Tissue
  • 07:51DNA Extraction
  • 09:19Planting of Seedlings with the Desired Genotyping
  • 10:14Representative Results Illustrating Arabidopsis Seedlings Grown Using the Ice-Cap System
  • 10:36Conclusion

Summary

Traduction automatique

O método Ice Cap-permite cultivar plantas em placas de 96 poços e não-destrutiva do tecido raiz colheita de cada muda. DNA extraído dessa raiz tecido pode ser usado para reações de genotipagem. Descobrimos que Ice Cap-funciona bem para<em> Arabidopsis thaliana</em>, Tomate, mudas e arroz.

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