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Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
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Neurosciences
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Journal JoVE Neurosciences
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

DOI:
10.3791/3288-v

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10:36 min

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February 07, 2012

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, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Chapitres

  • 00:05Titre
  • 02:49Sterilization, Priming and Testing of Patch-clamp Chips
  • 04:14Chips Preparation in Cell Biology Lab
  • 07:07Cell Plating of Snail Neurons
  • 08:45Electrophysiological Recordings
  • 09:09Representative Results for the Electrophysiology of Cells on NRCC Patch-clamp Chips
  • 09:22Conclusion

Summary

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We show how planar patch-clamp chips fabricated at the National Research Council of Canada are sterilized, primed, loaded with medium, plated with cells, and used for electrophysiological recordings.

Tags

CulturingElectrophysiologyCellsPatch-clampChipsSensitivityIon ChannelsGold StandardDisease ModelsPharmaceutical ScreensGlass PipetteMembrane PatchElectrodeIon-channel ActivityWhole CellPatch-clamp ChipsSuspended FilmAperture MicrofabricatedAutomated SystemsHigh-throughput ScreeningFluidic DeliverySuctionCell-to-probe SealsWhole Cell ModeSilicon Patch-clamp ChipImpedanceOrifice ShapeAction PotentialsSnail NeuronsMammalian Neurons

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