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FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability
Journal JoVE
Biologie
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Journal JoVE Biologie
FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

DOI:
10.3791/3289-v

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09:38 min

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August 05, 2011

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, , ,
1Biotechnology Research Institute,AUT University and KODE Biotech Ltd, 2Shemyakin Institute of Bioorganic Chemistry RAS,Moscow, Russia

Chapitres

  • 00:05Titre
  • 01:06Preparation of FSL Constructs
  • 01:49Insertion of FSL Construct(s) into Membranes
  • 02:39FSL-labeling Can Be Applied to a Diverse Variety of Cell Types
  • 09:24Conclusion

Summary

Traduction automatique

Traduction automatique

Function-Spacer-Lipid (FSL) constructs allow the surface characteristics of living cells and virions to be modified without loss of vitality. The method requires only simple contact of an FSL construct solution with a cell/virion and spontaneous and stable surface incorporation occurs.

Tags

FSL ConstructsBiological MarkersModify Cell SurfacesModify Virion SurfacesViabilityGenetic EngineeringCovalent AttachmentChemical MoietiesSurface ModificationHarmlessly Modify Cell SurfacesKODE TechnologyFunctional Head GroupSpacerLipid TailDispersible In WaterMembrane IncorporationSaccharidesBlood Group-related DeterminantsSialic AcidsHyaluronan PolysaccharidesFluorophoresBiotinRadiolabelsPeptides

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