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1Department of Biomedical Engineering,University of Wisconsin-Madison, 2Materials Science Program,University of Wisconsin-Madison, 3Department of Neurological Surgery,University of Wisconsin-Madison, 4Carbone Comprehensive Cancer Center and Center for Stem Cell and Regenerative Medicine,University of Wisconsin-Madison
Chapitres
- 00:05Titre
- 01:57BTSC Neurosphere Dissociation
- 03:00Fabrication of Bi-layered su-8 Master
- 04:42Molding of the PDMS Stamp
- 05:31Assembly of Microfluidic Devices and Cell Culturing
- 07:04Long-term Time-lapse Imaging of BTSC Migration with BioStation IM
- 07:46Time-lapse Recordings of Migrating BTSC
- 08:40Conclusion
En compartmentalizing mikroflödessystem enhet för att utreda cancer migrering stamceller beskrivs. Denna nya plattform skapar en livskraftig cellulär mikromiljö och möjliggör mikroskopisk visualisering av levande celler förflyttning. Mycket rörliga cancerceller isoleras för att studera molekylära mekanismer för aggressiv infiltration, vilket kan leda till mer effektiv framtida behandlingar.
Tags
Cancer Stem Cell MigrationCompartmentalizing Microfluidic DevicesLive Cell ImagingRecherche en cancérologieCellular MigrationAggressive Cancer Cell InvasionMetastasisTherapeutic ResistanceGlioblastoma Multiforme (GBM)Primary Malignant Adult Brain TumorGBM InfiltrationTumor MicroenvironmentCancer Cell Migration MechanismsBrain Tumor Stem Cell (BTSC)Therapeutic ResistanceRecurrenceCell Migratory MechanismsConventional Migration AssaysMicrofluidic DevicesSingle Cell Analysis
