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Chapitres
- 00:05Titre
- 02:02Procedural Flow Chart
- 03:19cDNA Synthesis
- 06:31End Repair, Paired End Library
- 07:37Adding ‘A’ Bases to the 3′ End of the DNA Fragments
- 08:31Adapter Ligation
- 10:32Size Selection/Gel Purification
- 11:20Evaluation of Single-Read and Paired-End Sequencing Libraries
- 14:18Conclusion
Her beskriver vi en metode til klargøring af både enkelt læser og parret ende Illumina mRNA-Seq sekvensering biblioteker til genekspressionsanalyse baseret på T7 lineær RNA forstærkning. Denne protokol kræver kun 10 nanogram at starte total RNA og genererer meget konsekvent biblioteker repræsenterer hele udskrifter.
Tags
MRNA-SeqIllumina LibrariesGlobal Gene ExpressionMutation AnalysisAllele-specific ExpressionGenome-wide AnalysesNon-sequenced GenomesTranscript Copy NumbersIllumina’s Genome AnalyzerPaired End SequencingAlternate Splice JunctionsInsertions And DeletionsDe Novo Transcriptome AssemblyLimited Starting MaterialNanograms Of Total RNAPCR AmplificationMinimal PCR Amplification
